Synthesis and characterization of ZnO nanoparticles using aqueous extract of Becium grandiflorum for antimicrobial activity and adsorption of methylene blue

Abstract Nanotechnology is a recent field of modern research dealing with synthesis, strategy and manipulation particle’s structure in size range 1–100 nm. This study introduces one the methods synthesis nanoparticles, i.e., green ZnO NPs using aqueous leaf extract Becium grandiflorum (AM: ‘Yedegamentisie’). The biomolecules plant (such as phenols, flavonoids, saponins, glycosides, steroids, tannins alkaloids) were used capping reducing agent during NPs. Response surface methodology coupled Box-Behnken design (RSM-BBD) was to optimize adsorption studies as-synthesized Then, characterized different spectroscopic microscopic instruments such UV–Vis spectroscopy, FTIR, XRD SEM–EDS consider its purity, shape crystallinity. analysis showed peaks 305–312 nm due FTIR availability phytochemicals at 490 cm −1 . Powder patterns confirmed formation phase pure wurtzite structures synthesized remove MB dye from solution by acting photocatalyst adsorbent well as, it also antimicrobial activity against two gram positive ( Staphylococcus epidermidis , aureus ) three negative Escherichia coli Klebsiella pneumonia Pseudomonas aeruginosa bacteria.